Enhanced Growth and Osteogenic Differentiation of Human Osteoblast-Like Cells on Boron
Lubica Grausova Growth and Differentiation of Cell Populations
Institute of Physiology, Academy of Sciences of the Czech Republic
Abstract:
Nanocrystalline diamond (NCD) films were deposited on silicon substrates by a microwave plasma-enhanced CVD process and doped with 133, 1000 and 6700 ppm of boron in the gas phase. The electrical resistivity of the films decreased from >10 MΩ (non-doped films) to 55, 0.6, and 0.3 kΩ (doped films with 133, 1000 and 6700 ppm of B, respectively). The increase in the number of human osteoblast-like MG 63 cells in 7-day-old cultures on NCD films was most apparent on NCD doped with 133 and 1000 ppm of B (152,500 ± 13,900 and 152,200 ± 10,400 cells/cm2, respectively, compared to 112,900 ± 9,700 cells/cm2 on non-doped NCD films). On NCD films with 6700 ppm of B, the cells contained the highest concentration of focal adhesion protein vinculin, measured per mg of protein.Also the concentration of osteocalcin, an important marker of osteogenic cell differentiation, increased with increasing level of B doping. The cell viability on all tested NCD films was almost 100%. Measurements of the concentration of ICAM-1, i.e. an immunoglobuline adhesion molecule binding inflammatory cells, suggested that the cells on the NCD films did not undergo significant immune activation. Therefore, the potential of NCD films for bone tissue regeneration can be further enhanced by boron-doping.
